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Growth of Haemophilus influenzae type b in continuous culture: Effect of dilution rate on outer-membrane protein and lipopolysaccharide expression
- Source :
- FEMS Microbiology Letters; May 1992, Vol. 93 Issue: 1 p43-43, 1p
- Publication Year :
- 1992
-
Abstract
- Haemophilus influenzae</it> type b (Hib) was grown in continuous culture under cystine-limitation between dilution rates (D) of 0.065–0.28 h−1. A similar outer-membrane protein profile, as adjudged by SDS-PAGE, was found at all dilution rates. However, a shift to a lipopolysaccharide structure with a greater electrophoretic mobility on SDS-PAGE with accompanying changes in monoclonal antibody reactivity was observed at D ≥ 0.15 h−1. Growth rate per se can affect the expression of outer-membrane components of Hib.
Details
- Language :
- English
- ISSN :
- 03781097 and 15746968
- Volume :
- 93
- Issue :
- 1
- Database :
- Supplemental Index
- Journal :
- FEMS Microbiology Letters
- Publication Type :
- Periodical
- Accession number :
- ejs35314300
- Full Text :
- https://doi.org/10.1111/j.1574-6968.1992.tb05038.x