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Dual effect of Thymosin α 1 on human monocyte-derived dendritic cell in vitrostimulated with viral and bacterial toll-like receptor agonists
- Source :
- Expert Opinion on Biological Therapy; July 2015, Vol. 15 Issue: 1, Supplement 1 p59-70, 12p
- Publication Year :
- 2015
-
Abstract
- Objectives:Thymosin α 1 (Tα1) recently gained interest as immune adjuvant for vaccines because of its ability to modulate the T-cell/dendritic cell (DC) axis and to improve antibody production. The objective of this study was to determine whether Tα1 would address in vitrothe response of human primary monocyte-derived DC, crucial regulators of vaccine-induced immunity, upon exposure to different toll-like receptor (TLR) agonists or infection with viruses or bacteria.Methods:DC maturation and production of pro-inflammatory cytokines were analyzed.Results:Our data revealed a dual effect of Tα1 on DC biology upon viral or bacterial stimulation. Interestingly, Tα1 enhanced human leukocyte antigen (HLA)-I and II surface expression and secretion of IL-6, TNF-α and IL-8 when DCs were treated with viral TLR3 and TLR7/8 agonists. Similarly, in pandemic H1N1 influenza A-infected DCs, Tα1 raised the expression of maturation markers and type I and III Interferon (IFN). In contrast, following bacterial TLR2 and 4 stimulation, as well as upon Bacillus Calmette–Guerin infection, the presence of Tα1 in DC cultures drastically lowered the analyzed cellular parameters.Conclusion:The knowledge that Tα1 pleiotropic effect might ameliorate anti-viral immune responses and, at the same time, dampen inflammation caused by bacterial infections could lay the groundwork for a more appropriate therapeutic application of this molecule.
Details
- Language :
- English
- ISSN :
- 14712598 and 17447682
- Volume :
- 15
- Issue :
- 1, Supplement 1
- Database :
- Supplemental Index
- Journal :
- Expert Opinion on Biological Therapy
- Publication Type :
- Periodical
- Accession number :
- ejs36393865
- Full Text :
- https://doi.org/10.1517/14712598.2015.1019460