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Fed-batch cultivation for the production of clavulanic acid by an immobilized Streptomyces clavuligerusmutant
- Source :
- World Journal of Microbiology and Biotechnology; December 2001, Vol. 17 Issue: 9 p869-872, 4p
- Publication Year :
- 2001
-
Abstract
- In order to obtain high productivity of clavulanic acid, a newly-introduced carrier, polyurethane pellet (PUP) Z97-020 was used for the immobilization process. In a stirred-tank bioreactor, batch cultivation by Streptomyces clavuligerusKK immobilized on PUP Z97-020 gave about 3100 mg of clavulanic acid per litre, representing an increase of 200% in productivity compared with that by fed-batch cultivation of free cells (1500 mg/l). However, the clavulanic acid produced rapidly decomposed due to the pH change during batch cultivation. Fed-batch cultivation by immobilized S. clavuligerusKK gave an excellent level of clavulanic acid up to 3250 mg/l, a productivity increase of 220% compared with that by fed-batch cultivation of free cells. These results suggest that immobilization with PUP Z97-020 is a more effective process for the production of clavulanic acid and that the maintenance of pH by fed-batch cultivation with glycerol as a limiting substrate prevents the clavulanic acid from decomposing during the fermentation.
Details
- Language :
- English
- ISSN :
- 09593993 and 15730972
- Volume :
- 17
- Issue :
- 9
- Database :
- Supplemental Index
- Journal :
- World Journal of Microbiology and Biotechnology
- Publication Type :
- Periodical
- Accession number :
- ejs37171894
- Full Text :
- https://doi.org/10.1023/A:1013895617923