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Properties of human epididymal sperm obtained from an alloplastic spermatocele: motility assessment and penetration of zona-free hamster oocytes in the presence and absence of caffeine
- Source :
- Fertility and Sterility; September 1985, Vol. 44 Issue: 3 p401-405, 5p
- Publication Year :
- 1985
-
Abstract
- Sperm were collected over a period of months from a human alloplastic spermatocele implanted at the corpus/caudal epididymal junction and were evaluated for their maturity, motility, and ability to capacitate and acrosome react, as assessed by the hamster zona-free oocyte sperm penetration assay (SPA). A mean of 11×106sperm were obtained with each aspiration, with 34% to 40% being mature, normal forms. Motility was poor; 15%±5% showing nonprogressive movement. SPA results were 22%±3% oocyte penetration. Addition of 7.5mM caffeine markedly enhanced motility and improved the SPA results. After 30 minutes’ exposure, the motility was 45%±5%, with all spermatozoa exhibiting progressive movement. This stimulation was maintained over a 24-hour period. When caffeine was present during the 2-hour preincubation for the SPA, penetration rates increased to 50%±10% (P<0.05). These results demonstrate that the poor-quality sperm retrieved from a human alloplastic spermatocele can be improved with exogenous stimulation and suggest that their fertilizing capacity may be enhanced by this treatment.
Details
- Language :
- English
- ISSN :
- 00150282 and 15565653
- Volume :
- 44
- Issue :
- 3
- Database :
- Supplemental Index
- Journal :
- Fertility and Sterility
- Publication Type :
- Periodical
- Accession number :
- ejs38718084
- Full Text :
- https://doi.org/10.1016/S0015-0282(16)48867-9