Cloning and molecular characterization of Myb transcription factors from Leymus(Poaceae: Trticeae)

Plant Myb transcription factors are crucial in regulating secondary metabolism, cellular morphogenesis and responses to hormone and stress signals. In this study, 19 Myb gene fragments were isolated from five Leymusspecies, three of which, LrMyb3, LmMyb8 and LmMyb6 were nearly complete open reading frames encoding putative LeymusMyb genes. Sequence and phylogenetic analysis indicated that all these putative Myb from Leymusrepresent plant typical R2R3-Myb genes. Compared with Myb gene in Arabidopsis thaliana, the 19 LeymusMyb gene fragments of the R2R3 region amino acid showed three different types, W-x (19) W-x (19) W-x (12) F-x (18) W-x (18) W, W-x (19) W-x (19) W-x (12) M-x (18) W-x (18) W and W-x (19) W-x (19) R-x (12) L-x (18) W-x (18) W. LmMyb8 gene shares high similarity to ZmMyb39 which was possible involvement of caffeic acid O-methyl-transferase (COMT) in the lignification process. TheLmMyb8 gene is expressed at high level in leaves and roots while at low level in stems.