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A preliminary report on the direct transfusion of blood in animals given excessive doses of diphtheria toxins

Authors :
Crile, George W.
Dolley, D. H.
Source :
Experimental Biology and Medicine; August 1906, Vol. 4 Issue: 1 p65-67, 3p
Publication Year :
1906

Abstract

Technique.— The dog was given subcutaneouslythe dose noted. After waiting a certain time an anastomosis was made between one of his vessels (usually, for convenience, the external jugular) and an artery (carotid) of a donor, of equal or usually larger size. When this was perfect, the toxic dog was bled, usually from a femoral artery, as rapidly as possible, to complete exsanguination, and the transfusion was in no case started till cessation of respiration gave warning of the limit's being reached. When this occurred the blood was allowed to flow, under control, until the pulse returned in every case to a better quantity than before. The time taken in transfusing was usually about 15 minutes. (The venous anastomosis was made because more blood went into the donee by it.)The experiments were next varied in this way; instead of treating a toxic dog with normal blood, an exsanguinated normal dog was transfused from one which had been given the toxin subcutaneously some time previous, as noted below. To be sure of an excess of toxin the dose was doubled. The technique was the same, under ether with careful asepsis. The vascular anastomosis was made before the normal dog was bled. The time is calculated from when the dose was given till the transfusion was started. In the fourth experiment one donor (St. Bernard dog weighing 40 k.) supplied three small dogs one after another with sufficient blood.No further observations of these dogs were made. The paralyzed dog is certainly suggestive of diphtheritic paralysis. The dog lived for over a month in this paralyzed state, but when he died I was not informed and the body was buried.

Details

Language :
English
ISSN :
15353702 and 15353699
Volume :
4
Issue :
1
Database :
Supplemental Index
Journal :
Experimental Biology and Medicine
Publication Type :
Periodical
Accession number :
ejs40757643
Full Text :
https://doi.org/10.3181/00379727-4-47