Interaction of a rat kidney endoplasmic reticulum fraction with glycolytic enzymes

The effects of glycolysis of a rat-kidney microsomal membrane preparation have been studied. The membrane preparation stimulates glycolytic activity more than 50%. The site of this stimulation is shown to be the 3-phosphoglycerate kinase reaction. Evidence is presented that the stimulation is due to ATPase activity of the membrane interacting with ATP associated with the kinase enzyme. The membrane preparation is shown to oxidize DPNH generated by glycolysis. The observed interaction between enzymes glycolysis and the membrane is interpreted as a channeling of metabolic energy into the membrane for active transport and demonstrates one mechanism whereby active transport stimulates a metabolic pathway.