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Multilocus Phylogeny and Antifungal Susceptibility of AspergillusSection Circumdatifrom Clinical Samples and Description of A. pseudosclerotiorumsp. nov

Authors :
Siqueira, J. P. Z.
Sutton, D. A.
Gené, J.
García, D.
Wiederhold, N.
Peterson, S. W.
Guarro, J.
Source :
Journal of Clinical Microbiology; February 2017, Vol. 55 Issue: 3 p947-958, 12p
Publication Year :
2017

Abstract

ABSTRACTA multilocus phylogenetic study was carried out to assess species identity of a set of 34 clinical isolates from Aspergillussection Circumdatifrom the United States and to determine their in vitroantifungal susceptibility against eight antifungal drugs. The genetic markers used were the internal transcribed spacer (ITS) region, and fragments of the beta-tubulin (BenA), calmodulin (CaM), and RNA polymerase II second largest subunit (RPB2) genes. The drugs tested were amphotericin B, itraconazole, posaconazole, voriconazole, anidulafungin, caspofungin, micafungin, and terbinafine. The most common species sampled was A. westerdijkiae(29.4%), followed by a novel species, which was described here as A. pseudosclerotiorum(23.5%). Other species identified were A. sclerotiorum(17.6%), A. ochraceus(8.8%), A. subramanianii(8.8%), and A. insulicolaand A. ochraceopetaliformis, with two isolates (5.9%) of each. The drugs that showed the most potent activity were caspofungin, micafungin, and terbinafine, while amphotericin B showed the least activity.

Details

Language :
English
ISSN :
00951137 and 1098660X
Volume :
55
Issue :
3
Database :
Supplemental Index
Journal :
Journal of Clinical Microbiology
Publication Type :
Periodical
Accession number :
ejs41408015
Full Text :
https://doi.org/10.1128/JCM.02012-16