Chromatographic Fingerprinting Analysis of Secondary Metabolites Present in Ethanolic Extract of Tabernaemontana divaricata(L.) R. Br. by HPTLC Technique

AbstractTabernaemontana divaricata(L.) R. Br is a medicinal plant which is blongs to the family of Apocyanaceae, the whole plant extract possess many biological activities such as antioxidant activity, antiinflammatory activity and it has a very good local anesthetic and anti cancer activity. Therefore, the present study is an attempt to evaluate the present of secondary metabolites from ethanolic extract of Tabernaemontana divaricata(L.) using HPTLC analysis which was carried out to find the alkaloids, flavonoids, glycosides, poly phenols, steroids and tannins. In the results, the selected secondary metabolites were present in the ethanolic extract of T. divaricata(L.). Based on the results, this study can be concluded that this plant has variety of biological activities may be presence of alkaloids, flavonoids, glycosides, poly phenols, steroids and tannins in it. In future by isolating and identifying these compounds may be formulated to treat the various diseases.