Identification of the active protein in rice bran protein having an inhibitory activity of cholesterol micellar solubility

In our previous study, rice bran protein (RBP) inhibited cholesterol micellar solubility in vitroand decreased serum cholesterol level in rats. In the present study, RBP was separated and purified by size-exclusion chromatography and reversed-phase chromatography. The active protein of RBP related to cholesterol micellar solubility was identified as lectin and non-specific lipid-transfer protein 1 using MALDI-TOF mass spectrometry analysis.