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The absence of Emp24p, a component of ER‐derived COPII‐coated vesicles, causes a defect in transport of selected proteins to the Golgi.

Authors :
Schimmöller, F.
Singer‐Krüger, B.
Schröder, S.
Krüger, U.
Barlowe, C.
Riezman, H.
Source :
EMBO Journal; April 1995, Vol. 14 Issue: 7 p1329-1339, 11p
Publication Year :
1995

Abstract

Emp24p is a type I transmembrane protein that is involved in secretory protein transport from the endoplasmic reticulum (ER) to the Golgi complex. A yeast mutant that lacks Emp24p (emp24 delta) is viable, but periplasmic invertase and the glycosylphosphatidyl‐inositol‐anchored plasma membrane protein Gas1p are delivered to the Golgi apparatus with reduced kinetics, whereas transport of alpha‐factor, acid phosphatase and two vacuolar proteins is unaffected. Oligomerization and protease digestion studies of invertase suggest that the selective transport phenotype observed in the emp24 delta mutant is not due to a defect in protein folding or oligomerization. Consistent with a role in ER to Golgi transport, Emp24p is a component of COPII‐coated, ER‐derived transport vesicles that are isolated from a reconstituted in vitro budding reaction. We propose that Emp24p is involved in the sorting and/or concentration of a subset of secretory proteins into ER‐derived transport vesicles.

Details

Language :
English
ISSN :
02614189 and 14602075
Volume :
14
Issue :
7
Database :
Supplemental Index
Journal :
EMBO Journal
Publication Type :
Periodical
Accession number :
ejs42736341
Full Text :
https://doi.org/10.1002/j.1460-2075.1995.tb07119.x