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Study of the Plasma Membrane Proteome Dynamics Reveals Novel Targets of the Nitrogen Regulation in Yeast*

Study of the Plasma Membrane Proteome Dynamics Reveals Novel Targets of the Nitrogen Regulation in Yeast*

Authors :
Villers, Jennifer
Savocco, Jérôme
Szopinska, Aleksandra
Degand, Hervé
Nootens, Sylvain
Morsomme, Pierre
Source :
Molecular and Cellular Proteomics (MCP Online); September 2017, Vol. 16 Issue: 9 p1652-1668, 17p
Publication Year :
2017

Abstract

Yeast cells, to be able to grow on a wide variety of nitrogen sources, regulate the set of nitrogen transporters present at their plasma membrane. Such regulation relies on both transcriptional and post-translational events. Although microarray studies have identified most nitrogen-sensitive genes, nitrogen-induced post-translational regulation has only been studied for very few proteins among which the general amino acid permease Gap1. Adding a preferred nitrogen source to proline-grown cells triggers Gap1 endocytosis and vacuolar degradation in an Rsp5-Bul1/2-dependent manner. Here, we used a proteomic approach to follow the dynamics of the plasma membrane proteome after addition of a preferred nitrogen source. We identified new targets of the nitrogen regulation and four transporters of poor nitrogen sources—Put4, Opt2, Dal5, and Ptr2—that rapidly decrease in abundance. Although the kinetics is different for each transporter, we found that three of them—Put4, Dal5, and Ptr2—are endocytosed, like Gap1, in an Rsp5-dependent manner and degraded in the vacuole. Finally, we showed that Gap1 stabilization at the plasma membrane, through deletion of Bul proteins, regulates the abundance of Put4, Dal5 and Ptr2.

Details

Language :
English
ISSN :
15359476 and 15359484
Volume :
16
Issue :
9
Database :
Supplemental Index
Journal :
Molecular and Cellular Proteomics (MCP Online)
Publication Type :
Periodical
Accession number :
ejs43083643
Full Text :
https://doi.org/10.1074/mcp.M116.064923