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Advanced easySTED microscopy based on two-photon excitation by electrical modulations of light pulse wavefronts

Advanced easySTED microscopy based on two-photon excitation by electrical modulations of light pulse wavefronts

Authors :
Otomo, Kohei
Hibi, Terumasa
Fang, Yi-Cheng
Hung, Jui-Hung
Tsutsumi, Motosuke
Kawakami, Ryosuke
Yokoyama, Hiroyuki
Nemoto, Tomomi
Source :
Biomedical Optics Express; June 2018, Vol. 9 Issue: 6 p2671-2680, 10p
Publication Year :
2018

Abstract

We developed a compact stimulated emission depletion (STED) two-photon excitation microscopy that utilized electrically controllable components. Transmissive liquid crystal devices inserted directly in front of the objective lens converted the STED light into an optical vortex while leaving the excitation light unaffected. Light pulses of two different colors, 1.06 and 0.64 μm, were generated by laser diode-based light sources, and the delay between the two pulses was flexibly controlled so as to maximize the fluorescence suppression ratio. In our experiments, the spatial resolution of this system was up to three times higher than that obtained without STED light irradiation, and we successfully visualize the fine microtubule network structures in fixed mammalian cells without causing significant photo-damage.

Details

Language :
English
ISSN :
21567085
Volume :
9
Issue :
6
Database :
Supplemental Index
Journal :
Biomedical Optics Express
Publication Type :
Periodical
Accession number :
ejs45608147