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Sesquiterpenoids from Chloranthus anhuiensiswith Neuroprotective Effects in PC12 Cells
- Source :
- Journal of Natural Products; June 2018, Vol. 81 Issue: 6 p1391-1398, 8p
- Publication Year :
- 2018
-
Abstract
- Glutamate-induced excitotoxicity plays a vital role in neurodegenerative diseases. Neuroprotection against excitotoxicity has been considered as an effective experimental approach for preventing and/or treating excitotoxicity-mediated diseases. In the present study, six new sesquiterpenoids (1–6) and 26 known compounds of this type (7–32) were isolated and characterized from the whole plants of Chloranthus anhuiensis. Chlorantolide A (1) is the first example of a 5,6-seco-germacrane-type sesquiterpenoid, while phacadinane E (2) is a rare 4,5-seco-cadinane-type sesquiterpenoid. The structures of the new compounds were determined by spectroscopic analysis and by calculations of electronic circular dichroism (ECD) spectra. Their neuroprotective effects in mediating glutamate-induced PC12 cell apoptosis were evaluated. Compound 26exhibited potent neuroprotective activity with an EC50value of 3.3 ± 0.9 μM. Using Hoechst 33258 staining, a caspase-3 activity assay, and Western blot analysis it was demonstrated that this compound reduces the apoptosis of PC12 cells through inhibition of caspase-3 activity, while activating the Akt signaling pathway.
Details
- Language :
- English
- ISSN :
- 01633864 and 15206025
- Volume :
- 81
- Issue :
- 6
- Database :
- Supplemental Index
- Journal :
- Journal of Natural Products
- Publication Type :
- Periodical
- Accession number :
- ejs45625495
- Full Text :
- https://doi.org/10.1021/acs.jnatprod.7b01076