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Metabolic engineering of Corynebacterium glutamicumfor production of sunscreen shinorine
- Source :
- Bioscience, Biotechnology, and Biochemistry; July 2018, Vol. 82 Issue: 7 p1252-1259, 8p
- Publication Year :
- 2018
-
Abstract
- AbstractUltraviolet-absorbing chemicals are useful in cosmetics and skin care to prevent UV-induced skin damage. We demonstrate here that heterologous production of shinorine, which shows broad absorption maxima in the UV-A and UV-B region. A shinorine producing Corynebacterium glutamicumstrain was constructed by expressing four genes from Actinosynnema mirumDSM 43827, which are responsible for the biosynthesis of shinorine from sedoheptulose-7-phosphate in the pentose phosphate pathway. Deletion of transaldolase encoding gene improved shinorine production by 5.2-fold. Among the other genes in pentose phosphate pathway, overexpression of 6-phosphogluconate dehydrogenase encoding gene further increased shinorine production by 60% (19.1 mg/L). The genetic engineering of the pentose phosphate pathway in C. glutamicumimproved shinorine production by 8.3-fold in total, and could be applied to produce the other chemicals derived from sedoheptulose-7-phosphate.
Details
- Language :
- English
- ISSN :
- 09168451 and 13476947
- Volume :
- 82
- Issue :
- 7
- Database :
- Supplemental Index
- Journal :
- Bioscience, Biotechnology, and Biochemistry
- Publication Type :
- Periodical
- Accession number :
- ejs45915788
- Full Text :
- https://doi.org/10.1080/09168451.2018.1452602