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Live Staphylococcus aureusand bacterial soluble factors induce different transcriptional responses in human airway cells
- Source :
- Physiological Genomics; February 2005, Vol. 20 Issue: 3 p244-255, 12p
- Publication Year :
- 2005
-
Abstract
- To characterize the response of respiratory epithelium to infection by Staphylococcus aureus(S. aureus), human airway cells were incubated for 1 to 24 h with a supernatant of a S. aureusculture (bacterial supernatant), then profiled with a pangenomic DNA microarray. Because an upregulation of many genes was noticed around 3 h, three independent approaches were then used to characterize the host response to a 3-h contact either with bacterial supernatant or with live bacteria: 1) a DNA microarray containing 4,200 sequence-verified probes, 2) a semiquantitative RT-PCR with a set of 537 pairs of validated primers, or 3) ELISA assay of IL-8, IL-6, TNFα, and PGE2. Among others, Fos, Jun, and EGR-1 were upregulated by the bacterial supernatant and by live bacteria. Increased expression of bhlhb2 and Mig-6, promoter regions which harbor HIF responding elements, was explained by an increased expression of the HIF-1α protein. Activation of the inducible form of cyclooxygenase, COX-2, and of the interleukins IL-1, IL-6, and IL-8, as well as of the NF-κB pathway, was observed preferentially in cells in contact with bacterial supernatant. Early infection was characterized by an upregulation of anti-apoptotic genes and a downregulation of pro-apoptotic genes. This correlated with a necrotic, rather than apoptotic cell death. Overall, this first global description of an airway epithelial infection by S. aureusdemonstrates a larger global response to bacterial supernatant (in term of altered genes and variation factors) than to exponentially growing live bacteria.
Details
- Language :
- English
- ISSN :
- 10948341 and 15312267
- Volume :
- 20
- Issue :
- 3
- Database :
- Supplemental Index
- Journal :
- Physiological Genomics
- Publication Type :
- Periodical
- Accession number :
- ejs46224791
- Full Text :
- https://doi.org/10.1152/physiolgenomics.00135.2004