Identification of lysine residue 199 of human serum albumin as a binding site for benzylpenicilloyl groups

Cyanogen bromide and tryptic fragments of penicilloylated serum albumin from penicillin‐treated patients were separated by HPLC. Determinations of benzylpenicilloyl (BPO) were performed on the different fractions. A BPO containing peptide was identified by its amino acid sequence and the bound BPO group was located on the lysine residue 199.