Back to Search Start Over

LH receptors in ovine corpora lutea in relation to various physiological states and effects PGF-2α on LH-induced steroidogenesis in vitro

Authors :
Evrard-Herouard, M.
de la Llosa-Hermier, M. P.
Martinet, J.
Mauleon, P.
de la Llosa, P.
Hermier, C.
Source :
Reproduction; January 1981, Vol. 61 Issue: 1 p225-233, 9p
Publication Year :
1981

Abstract

Summary.The LH binding properties (determined using tritiated methylated LH) and the in-vitro steroidogenic activity of CL from ewes in the oestrous cycle or early pregnancy (Day 18) were compared.No significant alteration in the Kdvalues was observed. However, the number of sites was maximal at Day 10 of the cycle and in early pregnant animals which had not been pregnant for at least 3 months (dry ewes). Non-lactating or suckling ewes had half the numbers of binding sites. The increase of the number of receptor sites was accompanied by a steroidogenic response at lower LH concentration.During incubation or superfusion for 5 h, a refractoriness to LH stimulation appeared after 1 h with high LH concentrations and after 3 h with low concentrations. The opposite effect of the addition of indomethacin or PGF-2α suggests the intervention of PGs in this phenomenon.

Details

Language :
English
ISSN :
14701626
Volume :
61
Issue :
1
Database :
Supplemental Index
Journal :
Reproduction
Publication Type :
Periodical
Accession number :
ejs46985197
Full Text :
https://doi.org/10.1530/jrf.0.0610225