Interleukin-1α Stimulated Prostacyclin Release by Increasing Gene Transcription of Prostaglandin H Synthase and Phospholipase A2in Human Vascular Endothelial Cells

This study was conducted to evaluate the effects of interleukin-1α (IL-1α) on prostacyclin (PGI2) production in cultured human vascular endothelial cells in association with intracellular Ca2+, inositol 1,4,5-trisphosphate (IP3), and with prostaglandin H synthase (PGHS) and phospholipase A2(PLA2) gene expression by using the competitive polymerase chain reaction (PCR) method. IL-1α did not increase PGI2production for 15 min, but induced an increase of about three-fold relative to that in controls at 60 and 180 min. IL-1α had no effect on intracellular Ca2+levels throughout the experimental period. In this study, consistent with previous reports, PGHS-1 messenger RNA (mRNA) was constitutively expressed, whereas PLA2mRNA was not. After stimulation with IL-1α, PLA2mRNA level showed an eightfold increase within 15 min, and PGHS-2 mRNA level increased by 76-fold within 180 min. PGHS-1 mRNA level was increased 1.6-fold at 180 min. These results suggest the existence of regulatory mechanisms of IL-1α-induced PGI2production, which involve PGHS and PLA2gene transcription.