Back to Search
Start Over
Curcumin regulates the miR-21/PTEN/Akt pathway and acts in synergy with PD98059 to induce apoptosis of human gastric cancer MGC-803 cells
- Source :
- Journal of International Medical Research; March 2019, Vol. 47 Issue: 3 p1288-1297, 10p
- Publication Year :
- 2019
-
Abstract
- Objective PD98059 is a potent and selective inhibitor of mitogen-activated protein kinase. Substantial preclinical evidence has shown an anti-tumor effect of curcumin on various solid tumors. This study aimed to investigate whether curcumin synergistically acts with PD98059 in exerting anti-gastric cancer effects.Methods The cell counting kit-8 assay was used to detect cell proliferation of the human gastric cancer MGC-803 cell line. Flow cytometry was performed to detect apoptosis. Western blotting was used to detect phosphatase and tensin homolog (PTEN) and phosphorylated Akt (p-Akt) expression levels. Quantitative reverse transcription-polymerase chain reaction was used to determine microRNA-21 (miR-21).Results A dose of 5 to 40 µM curcumin inhibited proliferation of MGC-803 cells in a dose- and time-dependent manner. A high dose of curcumin strongly inhibited p-Akt protein expression. With increasing curcumin levels, PTEN expression increased and miR-21 levels decreased. These results suggest that curcumin negatively modulated the miR-21/PTEN/Akt pathway. Moreover, after pretreatment with PD98059, cell apoptosis induced by curcumin was significantly increased. Additionally, the inhibitory effects of curcumin on the miR-21/PTEN/Akt pathway were significantly enhanced.Conclusion PD98059 combined with curcumin may be a potential strategy for managing gastric cancer.
Details
- Language :
- English
- ISSN :
- 03000605 and 14732300
- Volume :
- 47
- Issue :
- 3
- Database :
- Supplemental Index
- Journal :
- Journal of International Medical Research
- Publication Type :
- Periodical
- Accession number :
- ejs49120830
- Full Text :
- https://doi.org/10.1177/0300060518822213