Effect of Cyclooxygenase Inhibition on Retinal and Choroidal Blood Flow during Hypercarbia in Newborn Piglets

The effect of the cyclooxygenase inhibitor, indomethacin, on choroidal (ChBF) and retinal (RBF) blood flow during hypercarbia was examined in 16 paralyzed and mechanically ventilated piglets less than 8 d old. The animals were randomly assigned to a control group (mean ± SEM: wt, 1.66 ± 0.1 kg; n= 8) that received a placebo infusion or to an indomethacin treatment group (wt, 1.68 ± 0.2 kg; n= 8) that received an infusion of indomethacin (5 mg/kg i.v. over 30 min). Baseline ChBF and RBF were measured using radiolabeled microspheres in room air before and 15 min after the administration of placebo or indomethacin. Animals were then exposed to 30 min of hypercarbia (6–7 CO2, arterial CO2pressure 8–10 kPa) and measurements were repeated. There were no significant differences in RBF between control (40 ± 3 mL/ min/100 g) and indomethacin-treated animals (40 ± 3 mL/ min/100 g) before administration of placebo or indomethacin. However, RBF decreased significantly in the indomethacin-treated animals (28 ± 2 mL/min/100 g) compared to the control group (42 ± 4 mL/min/100 g) 15 min after administration of placebo or indomethacin. Furthermore, an increase in RBF occurred during hypercarbia in the control group (86 ± 6 mL/min/100 g), but this change was blunted in the indomethacin-treated animals (33 ± 5 mL/min/100 g) (p< 0.001). In contrast, ChBF did not differ significantly between the control and indomethacin groups during the periods studied. These results suggest that the increase in RBF during hypercarbia is at least partially mediated by cyclooxygenase by-products of ara-chidonic acid metabolism. The change in ChBF appears to be less influenced by these mediators.