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Isolation and Identification of Alkaloids from Poisons of Fire Salamanders (Salamandra salamandra)
- Source :
- Journal of Natural Products; 20240101, Issue: Preprints
- Publication Year :
- 2024
-
Abstract
- Fire salamanders (Salamandra salamandra) are conspicuously colored amphibians secreting a skin poison that contains unique steroid alkaloids such as samandarine (1) and samadarone (2), exhibiting toxic as well as antimicrobial activities. Because of their antipredatory and anti-infectious functions, alkaloids from Salamandrapoison are of interest with regard to the threat that the lethal fungus Batrachochytrium salamandrivorans(Bsal) poses to salamanders. Nevertheless, reliable data on the biological activity of Salamandraalkaloids are scarce, in part due to the difficulty to obtain and study those substances. Thus, isolation of pure salamander alkaloids is an important task that might directly contribute to the understanding of Bsalinfections. Here we present a noninvasive isolation procedure for samandarine (1) and O-acetylsamandarine (3), as well as for two new alkaloids, O-3-hydroxybutanoylsamandarine (4) and samanone (6), using HPLC. For the first time, high-field NMR data are presented for these alkaloids. Analysis using GC/MS and ESI+-MS, provided important information on the structural variability of these salamander alkaloids.
Details
- Language :
- English
- ISSN :
- 01633864 and 15206025
- Issue :
- Preprints
- Database :
- Supplemental Index
- Journal :
- Journal of Natural Products
- Publication Type :
- Periodical
- Accession number :
- ejs50011304
- Full Text :
- https://doi.org/10.1021/acs.jnatprod.9b00065