IL10, TGF Beta1, and IFN Gamma Modulate Intracellular Signaling Pathways and Cytokine Production to Control Toxoplasma gondiiInfection in BeWo Trophoblast Cells1

Considering that interleukin 10 (IL10), transforming growth factor beta1 (TGFB1), and interferon gamma (IFNG) are involved in the susceptibility of BeWo trophoblast cells to Toxoplasma gondiiinfection, the aim of the present study was to investigate the effector mechanisms triggered by these cytokines in the control of T. gondiiin BeWo cells. For this purpose, infected/uninfected BeWo cells were treated with IL10, TGFB1 (50 ng/ml), and IFNG (20 or 100 ng/ml) in order to verify the phosphorylation of signal transducers and activators of transcription 1 (STAT1), STAT3, and Smad2, parasite intracellular proliferation, as well as the Th1/Th2/IL17A cytokine production. The treatment of BeWo cells with IL10 and TGFB1 favored T. gondiiproliferation, and these findings were associated with STAT3 and Smad2 phosphorylation, respectively (P <0.05). Also, these cytokine treatments were able to down-modulate TNF alpha (TNFA) and IL6 production (P <0.05). Low concentration of IFNG was unable to control T. gondiiinfection but was able to trigger STAT1 phosphorylation and up-regulate IL6 and IL17A production; whereas a high concentration of IFNG was unable to activate STAT1 but down-modulated IL6 and TNFA and increased T. gondiiproliferation (P <0.05). IL10, TGFB1, and IFNG regulate a differential T. gondiiproliferation in BeWo cells because they distinctly trigger intracellular signaling pathways and cytokine production, especially IL6 and TNFA. Our data open new windows to understand the mechanisms triggered by IL10, TGFB1, and IFNG at the maternal-fetal interface in the presence of T. gondii,contributing to recognizing the importance of these effector mechanisms involved in the vertical transmission of this parasite.