New species of Tulasnellaassociated with terrestrial orchids in Australia

Recent studies using sequence data from eight sequence loci and coalescent-based species delimitation methods have revealed several species-level lineages of Tulasnellaassociated with the orchid genera Arthrochilus, Caleana, Chiloglottis, and Drakaeain Australia. Here we formally describe three of those species, Tulasnella prima, T. secunda, and T. warcupiispp. nov, as well as an additional Tulasnellaspecies associated with Chiloglottisgrowing in Sphagnum, T sphagnetisp. nov. Species were identified by phylogenetic analyses of the ITS with up to 1.3 % sequence divergence within taxa and a minimum of 7.6 % intraspecific divergence. These new Tulasnella (Tulasnellaceae, Cantharellales)species are currently only known from orchid hosts, with each fungal species showing a strong relationship with an orchid genus. In this study, T. primaand T. sphagnetiassociate with Chiloglottis, while T. secundaassociates with Drakaeaand Caleana, and T. warcupiiassociates with Arthrochilus oreophilus.