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Flow Cytometry Characterization of Cerebrospinal Fluid Monocytes in Patients With Postoperative Cognitive Dysfunction: A Pilot Study

Authors :
Berger, Miles
Murdoch, David M.
Staats, Janet S.
Chan, Cliburn
Thomas, Jake P.
Garrigues, Grant E.
Browndyke, Jeffrey N.
Cooter, Mary
Quinones, Quintin J.
Mathew, Joseph P.
Weinhold, Kent J.
Amundsen, Cindy L.
Bengali, Shahrukh
Brigman, Brian E.
Bullock, W. Michael
Carter, Jessica
Chapman, Joseph
Cheong Yee Ching, Vanessa
Cohen, Harvey J.
Colin, Brian
D’Amico, Thomas A.
Devinney, Michael J.
DeOrio, James K.
Ellet, Tressa
Esclamado, Ramon M.
Ferrandino, Michael N.
Gadsden, Jeffrey
Guercio, Jason
Habib, Ashraf
Harpole, David H.
Hartwig, Mathew G.
Iboaya, Ehimemen
Inman, Brant A.
Khan, Anver
Lagoo-Deenadayalan, Sandhya
Lee, Paula S.
Lee, Walter T.
Lemm, John
Levinson, Howard
Mantyh, Christopher
McDonagh, David L.
Migaly, John
Mithani, Suhail K.
Moretti, Eugene
Moul, Judd W.
Newman, Mark F.
Ni, Katherine
Ohlendorf, Brian
Perez, Alexander
Peterson, Andrew C.
Ponussamy, Vikram
Preminger, Glenn M.
Robertson, Cary N.
Roman, Sanziana A.
Runyon, Scott
Sandler, Aaron
Scheri, Randall P.
Smith, S. Kendall
Talbot, Leonard
Thacker, Julie K. M.
Tong, Betty C.
Tu, Alexander
Vaslef, Steven N.
Waldron, Nathan
Wang, Xueyuan
Whitson, Heather
Wickenheisser, Victoria
Young, Christopher
Source :
Anesthesia & Analgesia; November 2019, Vol. 129 Issue: 5 pe150-e154, 5p
Publication Year :
2019

Abstract

Animal models suggest postoperative cognitive dysfunction may be caused by brain monocyte influx. To study this in humans, we developed a flow cytometry panel to profile cerebrospinal fluid (CSF) samples collected before and after major noncardiac surgery in 5 patients ≥60 years of age who developed postoperative cognitive dysfunction and 5 matched controls who did not. We detected 12,654 ± 4895 cells/10 mL of CSF sample (mean ± SD). Patients who developed postoperative cognitive dysfunction showed an increased CSF monocyte/lymphocyte ratio and monocyte chemoattractant protein 1 receptor downregulation on CSF monocytes 24 hours after surgery. These pilot data demonstrate that CSF flow cytometry can be used to study mechanisms of postoperative neurocognitive dysfunction.

Details

Language :
English
ISSN :
00032999 and 15267598
Volume :
129
Issue :
5
Database :
Supplemental Index
Journal :
Anesthesia & Analgesia
Publication Type :
Periodical
Accession number :
ejs51230116
Full Text :
https://doi.org/10.1213/ANE.0000000000004179