Back to Search
Start Over
A Millifluidic Device with Embedded Cross-Linked Enzyme Aggregates for Degradation of H2O2
- Source :
- ACS Applied Materials & Interfaces; February 2020, Vol. 12 Issue: 5 p6768-6775, 8p
- Publication Year :
- 2020
-
Abstract
- Millifluidic devices decorated with enzymes have been used for enzymatic reactions in continuous processes, but low enzymatic activity and enzyme leaching remain as challenges. Herein, we develop a strategy to embed cross-linked enzyme aggregates (CLEAs) on the surfaces of millifluidic devices to achieve higher enzymatic activity and better stability. Catalase was chosen as a model enzyme to degrade H2O2in wastewater samples. First, CLEA of catalase (153 ± 10 nm) was formed by simultaneous precipitation and cross-linking with 25.0 wt % acetonitrile containing 0.025 wt % glutaraldehyde in a millifluidic device. To immobilize CLEA, we first swell a piece of plastic tubing by using 5.0 wt % acetonitrile and then immerse it in an aqueous solution with 5.0 wt % (3-aminopropyl)triethoxysilane (APTES) and 5.0 wt % dextran polyaldehyde (DPA) subsequently. After CLEA is absorbed inside the expanded polymer network of the tubing, the tubing is tightened by using a vacuum to secure the immobilized CLEA. The millifluidic device decorated with CLEA of catalase has total activity of 660 U for degradation of H2O2, and it shows good stability under a flow rate of 200 μL/min. The tubing can be used to degrade 0.1 wt % H2O2solution continuously for 3 h or remove 2 wt % residual H2O2in wastewater for 2 h. The technique is general enough and can be applied to other types of enzymes for continuous enzymatic reactions.
Details
- Language :
- English
- ISSN :
- 19448244
- Volume :
- 12
- Issue :
- 5
- Database :
- Supplemental Index
- Journal :
- ACS Applied Materials & Interfaces
- Publication Type :
- Periodical
- Accession number :
- ejs52107155
- Full Text :
- https://doi.org/10.1021/acsami.9b21480