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Quantification of Bioactive Marker β-Amyrin by Validated High-Performance Thin-Layer Chromatographic- Densitometric Method in Different Species of MaytenusGrown in Saudi Arabia
- Source :
- Journal of Planar Chromatography - Modern TLC; December 2013, Vol. 26 Issue: 6 p475-479, 5p
- Publication Year :
- 2013
-
Abstract
- A simple and sensitive high-performance thin-layer chromatographic (HPTLC)-densitometric method was developed and validated for quantification of β-amyrin in the crude extracts of two species of Maytenus(Maytenus obscuraand Maytenus parviflora) grown in Saudi Arabia. HPTLC-densitometry was performed on glass-backed silica gel 60 F254TLC plates with the binary mobile phase hexane-ethyl acetate (3:1, v/ v). The developed plate was derivatized with p-anisaldehyde, then scanned and quantified densitometrically at 550 nm. The system was found to give a compact spot for β-amyrin at RFvalue of 0.38 ± 0.01. The method was found to be satisfactory in terms of sensitivity, accuracy, precision, and recovery. The content of β-amyrin was estimated as 0.42% ± 0.01% and 0.88% ± 0.01% w/ win M. obscuraand M. parviflora, respectively. The developed HPTLC technique can be very useful for the quantification of β-amyrin present in various medicinal plants.
Details
- Language :
- English
- ISSN :
- 09334173
- Volume :
- 26
- Issue :
- 6
- Database :
- Supplemental Index
- Journal :
- Journal of Planar Chromatography - Modern TLC
- Publication Type :
- Periodical
- Accession number :
- ejs52797606
- Full Text :
- https://doi.org/10.1556/JPC.26.2013.6.3