Determining the Development of Persisters in Extensively Drug-Resistant Acinetobacter baumanniiupon Exposure to Polymyxin B-Based Antibiotic Combinations Using Flow Cytometry

Polymyxin B-based combinations are increasingly prescribed as a last-line option against extensively drug-resistant (XDR) Acinetobacter baumannii. It is unknown if such combinations can result in the development of nondividing persister cells in XDR A. baumannii. We investigated persister development upon exposure of XDR A. baumanniito polymyxin B-based antibiotic combinations using flow cytometry.