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Effect of Prostaglandin D2on mRNA Expression of Three Isoforms of Hyaluronic Acid Synthase in Nasal Polyp Fibroblasts

Authors :
Hirata, Yuji
Kariya, Shin
Kanai, Kengo
Fujiwara, Tazuko
Makihara, Sei-ichiro
Omichi, Ryotaro
Higaki, Takaya
Haruna, Takenori
Oka, Aiko
Nishizaki, Kazunori
Okano, Mitsuhiro
Source :
American Journal of Rhinology & Allergy; January 2021, Vol. 35 Issue: 1 p44-51, 8p
Publication Year :
2021

Abstract

Background Hyaluronan is one of the major extracellular matrixes in chronic rhinosinusitis (CRS) associated with tissue remodeling. Prostaglandin D2(PGD2) is also associated with the pathogenesis of CRS. However, little is known about whether PGD2regulates hyaluronan production by human airway fibroblasts.Objective We sought to determine the effect of PGD2on the mRNA expression of three isoforms of membrane-bound hyaluronic acid synthase (HAS1, HAS2 and HAS3) in fibroblasts, the major source of hyaluronan production, derived from CRS patients.Methods Nasal polyp-derived fibroblasts (NPDF) and uncinate tissue-derived fibroblasts (UTDF) were established from CRS patients with nasal polyps and those without, respectively. These fibroblasts were stimulated with PGD2or PGD2receptor (DP/CRTH2)-selective agonists in the presence or absence of receptor-selective antagonists. mRNA levels for HAS1, HAS2 and HAS3 were determined by real-time quantitative PCR.Results PGD2(1 µM) significantly enhanced HAS1 but not HAS2 or HAS3 mRNA expression by NPDF. Enhanced HAS1 mRNA expression was also obtained by stimulation with a DP receptor-selective agonist, but not with a CRTH2 receptor-selective agonist. In addition, PGD2-induced HAS1 mRNA expression was significantly inhibited by pre-treatment with DP receptor-selective antagonists. Similar induction of PGD2-induced HAS1 mRNA expression was seen in UTDF.Conclusion PGD2selectively stimulates HAS1 mRNA expression in local fibroblasts in CRS via DP, but not CRTH2, receptors.

Details

Language :
English
ISSN :
19458924 and 19458932
Volume :
35
Issue :
1
Database :
Supplemental Index
Journal :
American Journal of Rhinology & Allergy
Publication Type :
Periodical
Accession number :
ejs54907959
Full Text :
https://doi.org/10.1177/1945892420932781