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Multiple Classes of Antimicrobial Peptides in Amaranthus tricolorRevealed by Prediction, Proteomics, and Mass Spectrometric Characterization
- Source :
- Journal of Natural Products; February 2021, Vol. 84 Issue: 2 p444-452, 9p
- Publication Year :
- 2021
-
Abstract
- Traditional medicinal plants are rich reservoirs of antimicrobial agents, including antimicrobial peptides (AMPs). Advances in genomic sequencing, in silico AMP predictions, and mass spectrometry-based peptidomics facilitate increasingly high-throughput bioactive peptide discovery. Herein, Amaranthus tricoloraerial tissue was profiled via MS-based proteomics/peptidomics, identifying AMPs predicted in silico. Bottom-up proteomics identified seven novel peptides spanning three AMP classes including lipid transfer proteins, snakins, and a defensin. Characterization via top-down peptidomic analysis of Atr-SN1, Atr-DEF1, and Atr-LTP1 revealed unexpected proteolytic processing and enumerated disulfide bonds. Bioactivity screening of isolated Atr-LTP1 showed activity against the high-risk ESKAPE bacterial pathogens (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, and Enterobacter cloacae). These results highlight the potential for integrating AMP prediction algorithms with complementary -omics approaches to accelerate characterization of biologically relevant AMP peptidoforms.
Details
- Language :
- English
- ISSN :
- 01633864 and 15206025
- Volume :
- 84
- Issue :
- 2
- Database :
- Supplemental Index
- Journal :
- Journal of Natural Products
- Publication Type :
- Periodical
- Accession number :
- ejs55635736
- Full Text :
- https://doi.org/10.1021/acs.jnatprod.0c01203