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Mammalian α-Keto Acid Dehydrogenase Complexes

Authors :
Tanaka, Nobuyuki
Koike, Kichiko
Hamada, Minoru
Otsuka, Kin-Ichi
Suematsu, Tadashi
Koike, Masahiko
Source :
Journal of Biological Chemistry; June 1972, Vol. 247 Issue: 12 p4043-4049, 7p
Publication Year :
1972

Abstract

The nonionic detergent Triton X-100 has been found to solubilize readily the pig heart 2-oxoglutarate dehydrogenase complex from the protamine precipitate eluate. A highly active preparation was obtained from the Triton X-100 extract by calcium phosphate gel-cellulose column chromatography.

Details

Language :
English
ISSN :
00219258 and 1083351X
Volume :
247
Issue :
12
Database :
Supplemental Index
Journal :
Journal of Biological Chemistry
Publication Type :
Periodical
Accession number :
ejs56173382
Full Text :
https://doi.org/10.1016/S0021-9258(19)45136-3