Back to Search Start Over

Identification, cloning, and characterization of Cherry Valley duck CD4 and its antiviral immune responses

Authors :
Li, Tianxu
Li, Rong
Zhang, Tingting
Zhang, Huihui
Song, Xingdong
Zhai, Xinyu
Wang, Jinchao
Xing, Bin
Hou, Xiaolan
Wei, Liangmeng
Source :
Poultry Science; September 2021, Vol. 100 Issue: 9
Publication Year :
2021

Abstract

CD4 protein is a single chain transmembrane glycoprotein and has a broad functionality beyond cell-mediated immunity. In this study, we cloned the full-length coding sequence (CDS) of duck CD4 (duCD4) and analyzed its sequence and structure, and expression levels in several tissues. It consists of 1,449 nucleotides and encodes a 482 amino acid protein. The putative protein of duCD4 consisted of an N-terminal signal peptide, three immunoglobulins and one immunoglobulins-like domain in its central, one terminal transmembrane region, and a C-terminal domain of the CD4 T cell receptor. The duCD4 also has the typical signature “CXC” of CD4s. The multiple sequence alignment suggests duCD4 has four potential N-glycosylation sites and the phylogenetic analysis suggests duCD4 shares greater similarity with avian than other vertebrates. Quantitative real-time PCR analysis showed that duCD4 mRNA transcripts are widely distributed in the healthy Cherry Valley duck, and the highest level in the thymus. During the virus infection, the obvious change of duCD4 expression was observed in the spleen, lung and brain, which suggesting that duCD4 could be involved in the host's immune response to multiple types of viruses. Our research studied the characterization, tissue distribution, and antiviral immune responses of duCD4.

Details

Language :
English
ISSN :
00325791 and 15253171
Volume :
100
Issue :
9
Database :
Supplemental Index
Journal :
Poultry Science
Publication Type :
Periodical
Accession number :
ejs56383974
Full Text :
https://doi.org/10.1016/j.psj.2021.101262