Ex Vivo Proliferation and Differentiation of CD34+Cells into Myeloid Progenitor Cells: Cord Blood Versus Mobilized Peripheral Blood.

The purpose of our study was to evaluate the capacities of cord blood (CB) CD34+cells to proliferate and differentiate ex vivo into myeloid lineage in response to cytokines and to compare them with mobilized peripheral blood (MPB) cells. Briefly, 2.5 × 104CD34+cells, isolated from CB (n=10) and MPB (n= 9), were cultured in 5 ml MacoBiotech HP01 (Macopharma) with SCF, Flt3-L, IL-3 and G-CSF. At day 9, 106cultured cells were replated for 5 additional days. Cells were counted and evaluated for their CD34, CD13 and CD15 expression. Differentiation into myeloid compartment was assessed by CD11b and CD16 coexpression on CD15+cells. We observed that