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Carrier Properties of a Protein Derived from Outer Membrane Protein A of Klebsiella pneumoniae
- Source :
- Infection and Immunity; November 1999, Vol. 67 Issue: 11 p5547-5551, 5p
- Publication Year :
- 1999
-
Abstract
- ABSTRACTWe have recently cloned a new protein, recombinant P40 (rP40). When tested in vivo after conjugation to a B-cell epitope, rP40 induces an important antibody response without the need for adjuvant. To characterize its potency, this carrier protein was coupled to a peptide derived from respiratory syncytial virus attachment G protein (G1′). After immunization of mice with the rP40-G1′ conjugate, strong antipeptide antibodies were detected, whereas peptide alone was not immunogenic. To emphasize the carrier properties of rP40, a polysaccharide derived from Haemophilus influenzaetype b (Hib) was coupled to it. Immunoglobulin G responses against the Hib polysaccharide were observed after coupling to rP40. Interestingly, an antipeptide antibody response was observed despite preexisting anti-rP40 antibodies generated by preimmunization with rP40. In addition, rP40 compares well with the reference carrier protein, tetanus toxoid (TT), since antibody responses of equal intensity were observed when a peptide or a polysaccharide was coupled to TT and rP40. Moreover, rP40 had advantages compared to TT; e.g., it induced a mixed Th1/Th2 response, whereas TT induced only a Th2 profile. Together, the results indicate that rP40 is a novel carrier protein with potential for use as an alternative carrier for human vaccination.
Details
- Language :
- English
- ISSN :
- 00199567 and 10985522
- Volume :
- 67
- Issue :
- 11
- Database :
- Supplemental Index
- Journal :
- Infection and Immunity
- Publication Type :
- Periodical
- Accession number :
- ejs57553638
- Full Text :
- https://doi.org/10.1128/IAI.67.11.5547-5551.1999