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Stimulation of beta(1----3)glucan synthetase of various fungi by nucleoside triphosphates: generalized regulatory mechanism for cell wall biosynthesis

Stimulation of beta(1----3)glucan synthetase of various fungi by nucleoside triphosphates: generalized regulatory mechanism for cell wall biosynthesis

Authors :
Szaniszlo, P J
Kang, M S
Cabib, E
Source :
Journal of Bacteriology; March 1985, Vol. 161 Issue: 3 p1188-1194, 7p
Publication Year :
1985

Abstract

Particulate fractions from the taxonomically diverse fungi Achlya ambisexualis, Hansenula anomala, Neurospora crassa, Cryptococcus laurentii, Schizophyllum commune, and Wangiella dermatitidis were found to catalyze the time-dependent incorporation of glucose from UDP-[14C]glucose into a water-insoluble material. The reaction was stimulated by bovine serum albumin. The product was characterized as beta(1----3)glucan on the basis of its resistance to alpha- and beta-amylase and susceptibility to beta(1----3)glucanase. With the exception of the preparation from A. ambisexualis, all others were stimulated by nucleoside triphosphates and their analogs. The best activators were GTP and guanosine 5'-(gamma-thio)triphosphate. It is concluded that the stimulation by nucleotides, previously found with the glucan synthetase of Saccharomyces cerevisiae, is a regulatory mechanism that was well conserved during fungal evolution, presumably because of its importance in controlling cell wall biosynthesis and cell growth.

Details

Language :
English
ISSN :
00219193 and 10985530
Volume :
161
Issue :
3
Database :
Supplemental Index
Journal :
Journal of Bacteriology
Publication Type :
Periodical
Accession number :
ejs57609834
Full Text :
https://doi.org/10.1128/jb.161.3.1188-1194.1985