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Stimulation of beta(1----3)glucan synthetase of various fungi by nucleoside triphosphates: generalized regulatory mechanism for cell wall biosynthesis
Stimulation of beta(1----3)glucan synthetase of various fungi by nucleoside triphosphates: generalized regulatory mechanism for cell wall biosynthesis
- Source :
- Journal of Bacteriology; March 1985, Vol. 161 Issue: 3 p1188-1194, 7p
- Publication Year :
- 1985
-
Abstract
- Particulate fractions from the taxonomically diverse fungi Achlya ambisexualis, Hansenula anomala, Neurospora crassa, Cryptococcus laurentii, Schizophyllum commune, and Wangiella dermatitidis were found to catalyze the time-dependent incorporation of glucose from UDP-[14C]glucose into a water-insoluble material. The reaction was stimulated by bovine serum albumin. The product was characterized as beta(1----3)glucan on the basis of its resistance to alpha- and beta-amylase and susceptibility to beta(1----3)glucanase. With the exception of the preparation from A. ambisexualis, all others were stimulated by nucleoside triphosphates and their analogs. The best activators were GTP and guanosine 5'-(gamma-thio)triphosphate. It is concluded that the stimulation by nucleotides, previously found with the glucan synthetase of Saccharomyces cerevisiae, is a regulatory mechanism that was well conserved during fungal evolution, presumably because of its importance in controlling cell wall biosynthesis and cell growth.
Details
- Language :
- English
- ISSN :
- 00219193 and 10985530
- Volume :
- 161
- Issue :
- 3
- Database :
- Supplemental Index
- Journal :
- Journal of Bacteriology
- Publication Type :
- Periodical
- Accession number :
- ejs57609834
- Full Text :
- https://doi.org/10.1128/jb.161.3.1188-1194.1985