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Investigation of apparent false-positive urine latex particle agglutination tests for the detection of group B streptococcus antigen

Authors :
Harris, M C
Deuber, C
Polin, R A
Nachamkin, I
Source :
Journal of Clinical Microbiology; October 1989, Vol. 27 Issue: 10 p2214-2217, 4p
Publication Year :
1989

Abstract

In our nursery, we identified neonates with positive urine latex particle agglutination (LPA) tests for group B streptococcus (GBS) antigen who did not have corroborating cultural evidence of infection. To investigate the mechanisms underlying these apparent false-positive reactions, we examined the urine LPA test in an unselected population of neonates suspected of sepsis. Urine specimens for LPA testing and culture and simultaneous perirectal cultures were obtained from 134 neonates with suspected sepsis. Six infants had positive blood cultures for GBS; four of the six were positive by LPA testing. An additional 20 infants had positive LPA tests but negative blood cultures; of these, 13 had mothers who received antibiotic treatment prior to delivery. Two infants with positive LPA results and negative blood cultures had bacteria isolated from urine cultures obtained in a nonsterile fashion (GBS, Escherichia coli). GBS was not isolated from perirectal swabs of infants with positive LPA tests and negative blood cultures. In conclusion, (i) a high proportion of neonates evaluated for sepsis gave positive LPA tests and negative blood cultures, (ii) local contamination of the perirectal skin or urinary tract with GBS was an unlikely source of false-positive LPA reactions, and (iii) maternal antibiotic pretreatment during labor may represent an important cause of apparent false-positive LPA reactions.

Details

Language :
English
ISSN :
00951137 and 1098660X
Volume :
27
Issue :
10
Database :
Supplemental Index
Journal :
Journal of Clinical Microbiology
Publication Type :
Periodical
Accession number :
ejs57717882
Full Text :
https://doi.org/10.1128/jcm.27.10.2214-2217.1989