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Regulation of the chondrocyte phenotype by β-catenin
- Source :
- Development; December 2002, Vol. 129 Issue: 23 p5541-5550, 10p
- Publication Year :
- 2002
-
Abstract
- β-Catenin regulates important biological processes, including embryonic development and tumorigenesis. We have investigated the role ofβ-catenin in the regulation of the chondrocyte phenotype. Expression ofβ-catenin was high in prechondrogenic mesenchymal cells, but significantly decreased in differentiated chondrocytes both in vivo and in vitro. Accumulation of β-catenin by the inhibition of glycogen synthase kinase-3β with LiCl inhibited chondrogenesis by stabilizing cell-cell adhesion. Conversely, the low level of β-catenin in differentiated articular chondrocytes was increased by post-translational stabilization during phenotypic loss caused by a serial monolayer culture or exposure to retinoic acid or interleukin-1β. Ectopic expression of β-catenin or inhibition of β-catenin degradation with LiCl or proteasome inhibitor caused de-differentiation of chondrocytes. Transcriptional activation ofβ-catenin by its nuclear translocation was sufficient to cause phenotypic loss of differentiated chondrocytes. Expression pattern of Jun, a known target gene of β-catenin, is essentially the same as that of β-catenin both in vivo and in vitro suggesting that Jun and possibly activator protein 1 is involved in the β-catenin regulation of the chondrocyte phenotype.
Details
- Language :
- English
- ISSN :
- 09501991 and 14779129
- Volume :
- 129
- Issue :
- 23
- Database :
- Supplemental Index
- Journal :
- Development
- Publication Type :
- Periodical
- Accession number :
- ejs59007343
- Full Text :
- https://doi.org/10.1242/dev.129.23.5541