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Anti-inflammatory, Analgesic, and Cytotoxic Effects of The Phytexponent: A Polyherbal Formulation
- Source :
- Journal of Evidence-Based Complementary & Alternative Medicine (JEBCAM); February 2022, Vol. 27 Issue: 1
- Publication Year :
- 2022
-
Abstract
- The Phytexponent is used to treat pain and inflammation in complementary and alternative medicine practices; however, empirical data supporting its pharmacological efficacy and safety is scanty, hence the present study. We used the carrageenan-induced paw oedema and the acetic acid-induced writhing techniques to determine the anti-inflammatory and analgesic efficacies, respectively, of the Phytexponent in Swiss albino mice models. The 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay technique was used to investigate the in vitrocytotoxic effects of the Phytexponent in the Vero E6 cell line. The Phytexponent exerted significant (P< .05) anti-inflammatory effects in the carrageenan-induced paw oedema mouse model in a dose- and time-dependent manner, with significantly higher efficacy at 250 mg/Kg BW, than indomethacin (4 mg/Kg BW), in the first, second, and third hour (P< .05). Besides, the Phytexponent significantly reduced the acetic acid-induced writhing frequency in mice (P< .05), in a dose-dependent manner, depicting its analgesic efficacy. Notably, the Phytexponent (at doses: 125 mg/Kg BW and 250 mg/Kg BW) exhibited significantly higher analgesic efficacy than the Indomethacin (P<.05). Moreover, the Phytexponent was not cytotoxic to Vero E6 cells (CC50>1000 µg/ml) compared to cyclophosphamide (CC50= 2.48 µg/ml). Thus, the Phytexponent has significant in vivoanti-inflammatory and analgesic efficacy in mice models and is not cytotoxic to Vero E6 cell line, depicting its therapeutic potential upon further empirical investigation.
Details
- Language :
- English
- ISSN :
- 21565872
- Volume :
- 27
- Issue :
- 1
- Database :
- Supplemental Index
- Journal :
- Journal of Evidence-Based Complementary & Alternative Medicine (JEBCAM)
- Publication Type :
- Periodical
- Accession number :
- ejs59062159
- Full Text :
- https://doi.org/10.1177/2515690X221082986