Mycobacterium tuberculosis Growth Inhibition by Constituents of Sapium haematospermum

Four novel compounds consisting of two new pimaranes, lecheronol A (<BO>1</BO>) and lecheronol B (<BO>2</BO>), an acylated cycloartane, 3-O-β-lauroyl-cycloart-(23E)-en-25-ol (<BO>10</BO>), and a highly oxygenated novel chalconoid, α,β,3,4,5,2‘,4‘,6‘-octahydroxydihydrochalcone (<BO>12</BO>), were isolated along with seven known triterpene derivatives and three flavonol glucosides from Mycobacterium tuberculosis growth-inhibiting fractions of the CH<INF>2</INF>Cl<INF>2</INF>/MeOH (1:1) extract of the aerial parts of Sapium haematospermum. Compounds <BO>1</BO>, <BO>3</BO> (3α-hydroxyolean-12-ene), <BO>8</BO> [3α-hydroxylup-20(29)-en], and <BO>9</BO> (cycloartanol) were found most active, with MIC values of 4, 12.2, 13.4, and 8 μg/mL, respectively. Cytotoxicity tests in Vero cells for compounds <BO>1</BO>, <BO>3</BO>, <BO>8</BO>, and <BO>9</BO> gave IC<INF>50</INF> values of 104.8, 127.2, 127.2, and 102.4 μg/mL, respectively.