Abstract 10258: Fibrin Gel Embedding Adipose-derived Mesenchymal Stem Cell Showed Therapeutic Effect Against Rat Ischemic Cardiomyopathy by Enhancement in Nox Synthesis

Introduction:Although transplantation of mesenchymal stem cell (MSC) is a promising therapeutic option for myocardial infarction, its? therapeutic effects are not satisfactory especially for severely damaged heart, which propose some modifications in cell therapy. One possibility may be how to enhance cytokine expression especially endothelial nitric oxide synthase (eNOS) based on cellular tissue transplantation.Hypothesis:In this study, we hypothesized that fibrin scaffold stimulates the NOx production in ADSC and the NOx may depend on the cardiac functional recovery after myocardial infarction.Methods and Results:In-vitro, ADSC embedded in fibrin-gel produced more eNOS and NO than 2-dimensionally cultured-ADSC did significantly (about 2.5 and 2 folds respectively).N(G)-nitro-L-arginine methyl ester (L-NAME), which is a non-selective NOS inhibitor, is added to ADSC and NOx production and cell viability were monitored. The cell viability was not changed and NOx production was almost completely blocked by L-NAME.In-vivo, transplantation of ADSC with or without L-NAME (group N and A respectively) on the heart surfaceor sham operation (group C) was performed in rats that were subjected to LAD ligation 2 weeks prior to the treatment (n=7 each).The number of transplant cell was 1x10^6/body. The cardiac function assessed with echocardiography was significantly better in group A than group N and C (EF:A, N and C group; 55%, 50% and 40%, p<0.05).Histological analysis revealed the capillary density in peri infarct area was increased and the cadiomyocyte dimeter was significantly decreased in A group compared with the other groups.Conclusion:Cellular tissue transplantation using fibrin scaffold and ADSCs enhanced NO production from ADSC in vitro and vivo examination, suggesting possibility in better clinical outcomes of cellular therapy for ischemic cardiomyopathy.