Abstract 13796: 1Hnmr Spectroscopy Reveals Only Subtle Differences Between Serum Metabolome of Metabolically Healthy Obese Subjects and Subjects With Metabolic Syndrome

Introduction:Obesity is a component of metabolic health assessment and metabolic syndrome (MetS) diagnosis. In recent years, concept of "metabolically healthy obese" (MHO) emerged to describe obese patients, not meeting the MetS diagnostic criteria in contrast to metabolically unhealthy obese (MUO) subjects. The aim of this study was to investigate the differences between metabolome of MHO, MUO and healthy slim (HS) subjects enrolled to MAGNETIC (Metabolic and Genetic Profiling of Young Adults with and without a Family History of Premature Coronary Heart Disease) study.Methods:This analysis comprised on 94 men (MUO n=24, MHO n=24, HS n=46) aged 18-35 years without any chronic disease requiring pharmacotherapy. Patients on dietary supplements, or being on any special diet were excluded from analysis. We performed metabolic profiling of serum spectra with the use of 1HNMR spectroscopy (Varian 600MHz). Obtained spectra were binned using intelligent adaptive binning algorithm. Principal component analysis and partial least square analysis discriminant analysis were carried out on binned spectra to identify metabolites that differed between groups. Variable in projection score (VIP score) >2.0 was considered significant.Results:The metabolic signature of MUO was mainly associated with obesity and insulin resistance. Branched chain amino acids concentration and their metabolites as well as kynurenine pathway metabolite concentrations were higher in MUO group. Metabolome of MHO showed features between the MUO and HS signature, but in general reflecting higher level of metabolites associated with obesity as compared with HS.Conclusions:The results of the present study revealed that metabolic signature of the MHO subjects is closely related to the same metabolic pathways, as in the MUO group, what undermines the concept of metabolically healthy obesity.