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Monitoring isoniazid metabolism in vivousing a near-infrared fluorescent probeElectronic supplementary information (ESI) available. See https://doi.org/10.1039/d2ay00185c

Authors :
Wang, Xiaoyan
Zhang, Juanjuan
Zhu, Jiaqi
Yuan, Zhenwei
Xu, Hui
Ran, Jianhua
Source :
Analytical Methods; 2022, Vol. 14 Issue: 23 p2284-2292, 9p
Publication Year :
2022

Abstract

As a strong nucleophilic substance, hydrazine is widely used in the fields of agriculture, industry, and medicine. Hydrazine compounds usually exist as intermediates of some drugs. Many drugs, such as isoniazid and carbidopa, produce hydrazine metabolites. Hydrazine is a genotoxic substance, which can cause DNA lesions and cancer vialong-term exposure. Therefore, it is very important to monitor the level of hydrazine in the human body with high selectivity and sensitivity. Here, we synthesized a near-infrared (NIR) fluorescent probe Cy-HZ based on the hemicyanine skeleton to visualize the metabolism of the drug isoniazid in vivo. The ester group of the probe reacts with hydrazine to generate Cy-H, causing a change in fluorescence. Here, we studied its absorption and fluorescence spectra, the recognition response to hydrazine, the imaging of exogenous hydrazine in cells and the imaging in mice and further applied the probe to monitor the distribution and metabolism of isoniazid.

Details

Language :
English
ISSN :
17599660 and 17599679
Volume :
14
Issue :
23
Database :
Supplemental Index
Journal :
Analytical Methods
Publication Type :
Periodical
Accession number :
ejs59959155
Full Text :
https://doi.org/10.1039/d2ay00185c