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DNA OF BACTERIOPHAGE PM2: A CLOSED CIRCULAR DOUBLE-STRANDED MOLECULE

DNA OF BACTERIOPHAGE PM2: A CLOSED CIRCULAR DOUBLE-STRANDED MOLECULE

Authors :
Espejo, Romilio T.
Canelo, Eliana S.
Sinsheimer, Robert L.
Source :
Proceedings of the National Academy of Sciences of the United States of America; August 1969, Vol. 63 Issue: 4 p1164-1168, 5p
Publication Year :
1969

Abstract

The DNA molecules isolated from mature bacteriophage PM2 are closed double-stranded rings of a molecular weight of six million. Direct evidence for the circularity and supercoiling of PM2 DNA was obtained by electron microscopy. Other properties, such as sedimentation behavior at neutral and alkaline pH, unusually high buoyant density in alkaline CsCl, and stability upon heating, are consistent with this structure. Only the four common nucleotides are found after hydrolysis and chromatographic analysis of PM2 DNA.

Details

Language :
English
ISSN :
00278424 and 10916490
Volume :
63
Issue :
4
Database :
Supplemental Index
Journal :
Proceedings of the National Academy of Sciences of the United States of America
Publication Type :
Periodical
Accession number :
ejs60445014
Full Text :
https://doi.org/10.1073/pnas.63.4.1164