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Enhanced Sensitivity of Detection of Cytotoxic T Lymphocyte Responses to HIV Type 1 Proteins Using an Extended In Vitro Stimulation Period for Measuring Effector Function in Volunteers Enrolled in an ALVAC-HIV Phase I/II Prime Boost Vaccine Trial in Thailand
- Source :
- AIDS Research and Human Retroviruses; June 01, 2004, Vol. 20 Issue: 6 p642-644, 3p
- Publication Year :
- 2004
-
Abstract
- A phase I/II prime-boost vaccine trial in HIV-1-seronegative adults was conducted in Thailand using ALVAC-HIV (vCP1521) as a prime, boosting with either oligomeric gp160 TH023/LAI or Chiron HIV Thai subtype E (CM235) plus U. S. subtype B (SF2) gp120. Cytotoxic T lymphocyte (CTL) assays were conducted at one of the vaccine trial sites (Siriraj Hospital) at a single time point following the completion of immunization demonstrated that 8 of 50 (16%) vaccine recipients showed HIV-specific CTL by standard chromium release assay (CRA) after in vitro stimulation (IVS) for 2 weeks. Five additional vaccinees (13/50 = 26%) showed CTL responses after IVS for up to 4 weeks. Moreover, one volunteer with a positive CTL response to a single HIV antigen at Day 14 demonstrated a response to an additional HIV-1 antigen(s) after the longer IVS period. CTL activity was CD8+ restricted. Despite extension of the IVS up to 4 weeks, no CTL responses were detected in placebo recipients. These results imply that extension of the IVS period may increase the sensitivity of the CRA when measuring HIV-specific CTL in ALVAC-HIV prime-boost recipients without compromising specificity.
Details
- Language :
- English
- ISSN :
- 08892229 and 19318405
- Volume :
- 20
- Issue :
- 6
- Database :
- Supplemental Index
- Journal :
- AIDS Research and Human Retroviruses
- Publication Type :
- Periodical
- Accession number :
- ejs6137050
- Full Text :
- https://doi.org/10.1089/0889222041217473