Back to Search Start Over

Evaluation of barcoded magnetic bead–based immunoassays for the simultaneous detection of feline leukemia virus antigen and antibody against feline immunodeficiency virus

Evaluation of barcoded magnetic bead–based immunoassays for the simultaneous detection of feline leukemia virus antigen and antibody against feline immunodeficiency virus

Authors :
Esty, Kathy
Elmer, Haylea
Nassar, Salam
Ruggiero, Anthony
Singh, Seema
Beall, Melissa
Buch, Jesse
Source :
Journal of Veterinary Diagnostic Investigation; January 2023, Vol. 35 Issue: 1 p57-61, 5p
Publication Year :
2023

Abstract

Testing platforms that leverage automation, require minimal sample volume, and enable various tests to be performed simultaneously on a single sample have the potential to improve workflow and efficiency in veterinary diagnostic laboratories. We evaluated a barcoded magnetic bead (BMB) technology using established immunoassays for detection of feline leukemia virus (FeLV) p27 antigen and antibody against feline immunodeficiency virus (FIV). Analytical sensitivity, limit of blank, and limit of detection were used to establish a functional sensitivity of 1.00 ng/mL of inactivated FeLV antigen and 35.7 ng/mL of anti-FIV monoclonal antibody. Common interferents, such as hemoglobin, lipid, and bilirubin, were not found to interfere with the performance of the assay. Intra- and inter-assay CVs were <13% for both assays using manufactured samples. Using a set of 116 feline samples, the diagnostic accuracy of our multiplex assay was 100% compared to reference assays. Performance in a convenience set of 1,000 feline samples submitted to a commercial diagnostic laboratory revealed a proportion of positive results of 1.3% for FeLV and 3.7% for FIV. BMB technology should enable rapid screening of samples for various markers in a single immunoassay well.

Details

Language :
English
ISSN :
10406387 and 19434936
Volume :
35
Issue :
1
Database :
Supplemental Index
Journal :
Journal of Veterinary Diagnostic Investigation
Publication Type :
Periodical
Accession number :
ejs61404694
Full Text :
https://doi.org/10.1177/10406387221129259