Bioprospecting Secondary Compounds from Usnea Longissima

The objective of our study to identified and characterized the compounds from Usnea longisssimia.Lichen thallus was subjected for extraction to evaluate phytochemicals in different polarity solvents and extract yield also quantified. Chemical markers were identified in specific solvent system which was isolated by preparative. TLC and characterized through FTIR spectroscopy. Usnic acid was identified as a major marker along with Barbatic acid, Diffractaic acid, Divaricatic acid, and Fumarprotocetraic acid, respectively. The yield of usnic acid was found to be maximum in ethanol and yield varied from 1.84 to 5.06%, respectively. This study resulted that polarity based extraction affects the yield of extracts and isolation of specific compounds.