A rapid and effective method for obtaining ardicrenin from Ardisia crenataSims var. bicolorplants

Ardisia crenataSims var. bicolorhas high ornamental and medical value. To protect wild germplasm resources and obtain more ardicrenin, a plant regeneration system was established via calli, and ardicrenin content was quantified by reversed-phase high-performance liquid chromatography (RP-HPLC). The results showed that a Murashige and Skoog (MS) medium with 2,4-dichlorophenoxyacetic acid (2,4-D, 1.0 mg L−1) and kinetin (KT, 0.01 mg L−1) was suitable for callus induction, with an induction rate of 95.31 ± 1.62%; a MS medium with 2,4-D (0.5 mg L−1) and 6-benzylaminopurine (6-BA, 0.2 mg L−1) was suitable for callus multiplication, with a multiplication coefficient of 359.26; a MS medium with 6-BA (1.0 mg L−1) and 1-naphthaleneacetic acid (NAA, 0.5 mg L−1) was suitable for callus differentiation, with a differentiation rate of 89.36 ± 1.39%; and 1/2 MS with indole-3-butyric acid (IBA, 0.2 mg L−1) was suitable for rooting, with a rooting rate of 93.74 ± 1.38%. Additionally, garden soil: vermiculite: perlite (v: v: v = 1:1:1) was appropriate for acclimatization and transplanting, with a survival rate of 87.38%. RP-HPLC analysis revealed that the ardicrenin content in one-month-old plantlets was higher than that in one-year-old plants and was nearly the same as the content observed in three-year-old plants and two-month-old transplanted plantlets, especially in the roots (17.69 mg g−1DW, 1.77% DW). In summary, a rapid propagation system for A. crenataSims var. bicolorwas successfully established for the first time, and the roots of 1-month-old plantlets were used to effectively obtain ardicrenin.