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In vitrosynergistic antifungal evaluation through combination of F127-conjugated amphotericin B and curcumin-loaded micelles
- Source :
- Journal of Drug Delivery Science and Technology; 20240101, Issue: Preprints
- Publication Year :
- 2024
-
Abstract
- Amphotericin B (AmB) coupled with hydrophilic or amphiphilic polymers and combined with natural active products should be an effective way to improve biosafety and dose-reduced activity of AmB against C. albicans. In the study, F127-AmB conjugate was synthesized through O- and N-alkylation with AmB and F127 as starting materials, and its hemolytic, aggregation and release behavior were evaluated. Curcumin-loaded micelles (Cur-M) were prepared to study its synergistic effect with F127-AmB conjugate micelles. It was found that AmB in F127-AmB mainly existed as monomers without hemolysis, suggesting its good biosafety to host. The in vitrorelease of AmB from the F127-AmB conjugate micelles was slow and sustained, and similar release property was observed for Cur-M. The anticandidal activity of F127-AmB conjugate micelles alone was good and similar to that of native AmB alone. However, the F127-AmB conjugate micelles showed strong synergism with Cur-M with fractional inhibitory concentration index value of 0.0936. The combination of both Cur-M and F127-AmB conjugate micelles not only significantly boosted the biofilm inhibition activity of F127-AmB conjugate micelles, but also caused C. albicansapoptosis at low concentration. So, combination of the conjugated AmB micelles and Cur-loaded micelles was a noteworthy strategy to improve AmB's antifungal activity and safety.
Details
- Language :
- English
- ISSN :
- 17732247
- Issue :
- Preprints
- Database :
- Supplemental Index
- Journal :
- Journal of Drug Delivery Science and Technology
- Publication Type :
- Periodical
- Accession number :
- ejs65235676
- Full Text :
- https://doi.org/10.1016/j.jddst.2024.105398