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A simple and efficient in plantatransformation method based on the active regeneration capacity of plants
A simple and efficient in plantatransformation method based on the active regeneration capacity of plants
- Source :
- Plant Communications; April 2024, Vol. 5 Issue: 4
- Publication Year :
- 2024
-
Abstract
- Plant genetic transformation strategies serve as essential tools for the genetic engineering and advanced molecular breeding of plants. However, the complicated operational protocols and low efficiency of current transformation strategies restrict the genetic modification of most plant species. This paper describes the development of the regenerative activity–dependent in plantainjection delivery (RAPID) method based on the active regeneration capacity of plants. In this method, Agrobacterium tumefaciensis delivered to plant meristems via injection to induce transfected nascent tissues. Stable transgenic plants can be obtained by subsequent vegetative propagation of the positive nascent tissues. The method was successfully used for transformation of plants with strong regeneration capacity, including different genotypes of sweet potato (Ipomoea batatas), potato (Solanum tuberosum), and bayhops (Ipomoea pes-caprae). Compared with traditional transformation methods, RAPID has a much higher transformation efficiency and shorter duration, and it does not require tissue culture procedures. The RAPID method therefore overcomes the limitations of traditional methods to enable rapid in plantatransformation and can be potentially applied to a wide range of plant species that are capable of active regeneration.
Details
- Language :
- English
- ISSN :
- 25903462
- Volume :
- 5
- Issue :
- 4
- Database :
- Supplemental Index
- Journal :
- Plant Communications
- Publication Type :
- Periodical
- Accession number :
- ejs65239751
- Full Text :
- https://doi.org/10.1016/j.xplc.2024.100822