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Multisignal Biosensors Based on Mn Paramagnetic Relaxation and Nanocatalysis for Norovirus Detection
- Source :
- Analytical Chemistry; March 2024, Vol. 96 Issue: 10 p4031-4038, 8p
- Publication Year :
- 2024
-
Abstract
- A multisignal method for the sensitive detection of norovirus based on Mn paramagnetic relaxation and nanocatalysis was developed. This dual-modality sensing platform was based on the strong relaxation generated by cracked Au@MnO2nanoparticles (NPs) and their intrinsic enzyme-like activity. Ascorbic acid rapidly cracked the MnO2layer of Au@MnO2NPs to release Mn(II), resulting in the relaxation modality being in a “switch-on” state. Under the optimal conditions, the relaxation modality exhibited a wide working range (6.02 × 103–3.01 × 107copies/μL) and a limit of detection (LOD) of 2.29 × 103copies/μL. Using 4,4′,4″,4″′-(porphine-5,10,15,20-tetrayl) tetrakis (benzenesulfonic acid) (tpps)-β-cyclodextrin (tpps-β-CD) as a T1relaxation signal amplification reagent, a lower LOD was obtained. The colorimetric modality exploited the “peroxidase/oxidase-like” activity of Au@MnO2NPs, which catalyzed the oxidation of colorless 3,3′,5,5′-tetramethylbenzidine (TMB) to blue oxidized TMB, which exhibited a working range (6.02 × 104–6.02 × 106copies/μL) and an LOD of 2.6 × 104copies/μL. In addition, the rapid amplification reaction of recombinase polymerase enabled the detection of low norovirus levels in food samples and obtained a working range of 101–106copies/mL and LOD of 101copies/mL (relaxation modality). The accuracy of the sensor in the analysis of spiked samples was consistent with that of the real-time quantitative reverse transcription polymerase chain reaction, demonstrating the high accuracy and practical utility of the sensor.
Details
- Language :
- English
- ISSN :
- 00032700 and 15206882
- Volume :
- 96
- Issue :
- 10
- Database :
- Supplemental Index
- Journal :
- Analytical Chemistry
- Publication Type :
- Periodical
- Accession number :
- ejs65591544
- Full Text :
- https://doi.org/10.1021/acs.analchem.3c03950